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BIOL3137 Tools for Molecular Ecology

Later Year Course

Offered By School of Botany and Zoology
Academic Career Undergraduate
Course Subject Biology
Offered in First Semester, 2009 and First Semester, 2010
Unit Value 6 units
Course Description This course explores the essential theory underpinning the laboratory tools available for the molecular genetic analysis of individuals, populations and species of microorganisms, especially those causing disease. These methods are also applicable to the genetic analyses of non-disease causing agents e.g. plants and animals.

Topics include:

  • DNA extraction
  • RNA extraction
  • DNA fingerprinting
  • PCR
  • PCR based molecular markers including RFLP, AFLP, microsatellites, SNPs
  • DNA sequencing
  • Virulence profiling of microorganisms
  • Multi-locus sequence typing
  • DNA barcoding
  • Molecular ecology

The course will also review: the principles of laboratory safety, chemistry for general laboratory analysis and procedures essential for working with bacterial and fungal organisms.

Learning Outcomes

On satisfying the requirements of this course, students will have the knowledge and skills to:

1. demonstrate knowledge of the principles of laboratory safety
2. understand and describe the range of molecular genetic analysis tools available and evaluate their strengths and limitations for different tasks
3. perform laboratory work under supervision including DNA extraction, PCR and DNA sequencing
4. perform and analyse the outcomes of a laboratory-based mini project, and compile and interpret data generated by this work in written and oral form
Indicative Assessment

Assessment will be based on:

  • Theory quizzes (5%; LO 1-2)
  • Lab book assessment (20%; LO 1,3,4)
  • Assignment in form of practical report (40%; LO 1-4 )
  • Oral presentation (10%; LO 4)
  • Mid-term exam (25 %; LO 2-3)
Workload Up to 16 lectures, practical sessions and a large practical project
Requisite Statement BIOL2161 and BIOL2151
Recommended Courses BIOL3151
Science Group C
Academic Contact Dr Celeste Linde

The information published on the Study at ANU 2009 website applies to the 2009 academic year only. All information provided on this website replaces the information contained in the Study at ANU 2008 website.

Updated:   13 Nov 2015 / Responsible Officer:   The Registrar / Page Contact:   Student Business Solutions