This course provides an introduction to the principles and practice of recombinant DNA technology. The biochemical basis for each technique, as well as applications in medicine and agriculture, will be discussed. 

The following topics will be included: 

• DNA cloning; gene libraries
• DNA sequencing
• Polymerase chain reaction (PCR)
• Blotting techniques
• Expression of recombinant proteins
• Gene mapping
• Transgenic animals
• Gene therapy
• Combinatorial approaches 

These topics are fundamental to an understanding of biotechnology.

Note: Graduate students attend joint classes with undergraduates but are assessed separately.

On satisfying the requirements of this course, students will have the knowledge and skills to:

1. Describe and apply methods and techniques used to manipulate DNA.
2. Design strategies for identifying and analysing genes and their function.
3. Recognise the different requirements for gene expression in yeast, bacteria, plants and animals.
4. Demonstrate practical skills used in molecular biotechnology such as PCR and molecular cloning.
5. Obtain and evaluate information on a current topic in molecular biology and communicate this analysis in writing.

Assessment will be based on:

• Practical reports, tutorial test and assignments (total: 75%; LO 1, 2, 3, 4, 5)
• Final examination  (25%; LO 1, 2, 3)

Two lectures per week; ten laboratory and/or tutorial sessions of up to three hours each.

Bachelor degree; with second year Molecular Biology or Biochemistry and first year Chemistry.

Requires Bachelor of Science or equivalent with a major in the field of biological sciences with relevant experience or academic achievement; specific knowledge in the area of cell and molecular biology is required.

Brown, T.A. (2006) Gene Cloning and Analysis: an introduction (5th ed.) Blackwell