This course explores the essential theory underpinning the laboratory tools available for the molecular genetic analysis of individuals, populations and species of microorganisms, plants and animals. 
Topics include:

• DNA extraction
• RNA extraction
• DNA fingerprinting
• PCR
• PCR based molecular markers including RFLP, AFLP, microsatellites, SNPs
• DNA sequencing
• Virulence profiling of microorganisms
• Multi-locus sequence typing of microorganisms
• DNA bar-coding of plants and animals

The course will also review the principles of laboratory safety, chemistry for general laboratory analysis and procedures essential for working with bacterial and fungal organisms.

On satisfying the requirements of this course, students will have the knowledge and skills to:

1. Demonstrate knowledge of the principles of laboratory safety
2. Understand and describe the range of molecular genetic analysis tools available and evaluate their strengths and limitations for different tasks
3. Perform laboratory work under supervision including DNA extraction, PCR and DNA sequencing
4. Analyse the outcomes of a laboratory-based mini project, and compile and interpret data generated by this work in written and oral form

Assessment will be based on:

• Laboratory reports (40%; LO 1-4)
• Oral presentation (10%; LO 2-4)
• Quizzes (50%; LO 1, 2)

Bachelor of Science or equivalent with a major in the field of biological science with relevant experience or academic achievement. 

First year Biology, first year Genetics and first year Statistics are recommended. 

First year Chemistry is desirable.

Completion of BIOL6004 and BIOL6006

This course must be taken in conjunction with BIOL8211