This course will cover the theory and practice of light microscopy as applied in the biological sciences.  The light optical systems employed will be explained in detail, with particular emphasis on Köhler illumination and the processes involved confocal laser microscopy.  The concept of resolution limit, its calculation and significance will be highlighted.  The use of fluorescent stains to highlight cellular features will be demonstrated. The performance of digital cameras, recognition of image artefacts and methods to maximise signal-to-noise will be treated.

On satisfying the requirements of this course, students will have the knowledge and skills to: 

1.  understand the general principles behind fluorescence and confocal light microscopy techniques (LO1)

2. understand the different types of data that can be obtained using these microscopes; (LO2)

3. understand the advantages and limitations of different microscopic techniques for different types of samples and problems. (LO3)

1. Multiple-choice questions and written assignments for tutorials (20%); (LO 1,2,3)

2. Mid-term exam (20%); (LO1,2,3)

3. Assessable practicals (20%); and (LO 1,2,3)

4. A written report and oral presentation of second practical project (40%). (LO 1,2,3)

• Average weekly workload will be two hours of lectures;

• Four hours of associated reading per week; and

• One third of lectures will have online assessment and tutorials averaging a total of one hour per week.  

• Prerequisite is at least a Bachelors degree or equivalent work experience at the discretion of the Program Convener;

Knowledge of cell biology and molecular biology would be an advantage to students wanting to pursue biological microscopy.

To be confirmed.

Students must have access to computers with fast internet connection, webcams, , temporary LifeSize license (which we will arrange) and Skype in order to access remote learning resources. Access to light microscopes at their home laboratory is required for remote learning.