This course provides an introduction to the principles and practice of recombinant DNA technology. It has a focus on how biological processes can be exploited and manipulated for practical purposes, rather than how they operate in nature. The course also introduces some of the main problem solving skills used routinely in molecular biology. A wide range of methods and applications will be discussed including: DNA cloning, gene libraries, DNA sequencing, polymerase chain reaction (PCR), blotting techniques, expression of recombinant proteins, gene mapping, transgenic animals, and gene therapy.

On satisfying the requirements of this course, students will have the knowledge and skills to:

1. Describe and apply methods and techniques used to manipulate DNA and generate transgenic (genetically modified) organisms
2. Design strategies for identifying and analysing genes and their function
3. Recognise the different requirements for gene expression in yeast, bacteria, plants and animals
4. Demonstrate practical skills used in molecular biotechnology such as PCR and molecular cloning

Assessment will be based on:

• Tutorial tests (25%; LO 1, 2, 4)
• Practical lab exercises (journal and report 25%; LO 1, 3, 4)
• Final exam (50%; LO 1, 2, 3)

Two lectures per week; five laboratory sessions of up to four hours each; eight one-hour tutorials

BIOL2161 and CHEM1201 (or CHEM1015/17)

Brown, T.A. (2010) Gene Cloning and Analysis: an introduction (6th ed.) Wiley-Blackwell