This course explores the essential theory underpinning the laboratory tools available for the molecular genetic analysis of individuals, populations and species of microorganisms, especially those causing disease. These methods are also applicable to the genetic analyses of non-disease causing agents e.g. plants and animals.

Topics include:

• DNA extraction
• RNA extraction
• DNA fingerprinting
• PCR
• PCR based molecular markers including RFLP, AFLP, microsatellites, SNPs
• DNA sequencing
• Virulence profiling of microorganisms
• Multi-locus sequence typing
• DNA barcoding
• Molecular ecology

The course will also review: the principles of laboratory safety, chemistry for general laboratory analysis and procedures essential for working with bacterial and fungal organisms.

On satisfying the requirements of this course, students will have the knowledge and skills to:

1. demonstrate knowledge of the principles of laboratory safety
2. understand and describe the range of molecular genetic analysis tools available and evaluate their strengths and limitations for different tasks
3. perform laboratory work under supervision including DNA extraction, PCR and DNA sequencing
4. perform and analyse the outcomes of a laboratory-based mini project, and compile and interpret data generated by this work in written and oral form

Assessment will be based on:

• Theory quizzes (5%; LO 1-2)
• Lab book assessment (20%; LO 1,3,4)
• Assignment in form of practical report (40%; LO 1-4 )
• Oral presentation (10%; LO 4)
• Mid-term exam (25 %; LO 2-3)

Up to 16 lectures, practical sessions and a large practical project

BIOL2161 and BIOL2151

BIOL3151